pbs mcd4 Search Results


pbs mcd4  (Addgene inc)
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Addgene inc pbs mcd4
The epitope of ibalizumab is likely to be conformational. (A) Binding of ibalizumab to soluble hCD4, CD4-IgG2, PR14 (aa 121 to 134), or TG26 (aa 115 to 140) as measured by ELISA. (B) Competition for ibalizumab binding to sCD4 in solution by CD4-IgG2 or PR14 as measured by ELISA. (C) Binding titers of mouse anti-PR14 antiserum to hCD4 or PR14. (D) FACS analysis of cell surface staining by ibalizumab in control 293T cells or cells transfected with <t>hD2-mCD4</t> or hCD4 plasmid DNA.
Pbs Mcd4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The epitope of ibalizumab is likely to be conformational. (A) Binding of ibalizumab to soluble hCD4, CD4-IgG2, PR14 (aa 121 to 134), or TG26 (aa 115 to 140) as measured by ELISA. (B) Competition for ibalizumab binding to sCD4 in solution by CD4-IgG2 or PR14 as measured by ELISA. (C) Binding titers of mouse anti-PR14 antiserum to hCD4 or PR14. (D) FACS analysis of cell surface staining by ibalizumab in control 293T cells or cells transfected with hD2-mCD4 or hCD4 plasmid DNA.

Journal: Journal of Virology

Article Title: Epitope Mapping of Ibalizumab, a Humanized Anti-CD4 Monoclonal Antibody with Anti-HIV-1 Activity in Infected Patients ▿

doi: 10.1128/JVI.00453-10

Figure Lengend Snippet: The epitope of ibalizumab is likely to be conformational. (A) Binding of ibalizumab to soluble hCD4, CD4-IgG2, PR14 (aa 121 to 134), or TG26 (aa 115 to 140) as measured by ELISA. (B) Competition for ibalizumab binding to sCD4 in solution by CD4-IgG2 or PR14 as measured by ELISA. (C) Binding titers of mouse anti-PR14 antiserum to hCD4 or PR14. (D) FACS analysis of cell surface staining by ibalizumab in control 293T cells or cells transfected with hD2-mCD4 or hCD4 plasmid DNA.

Article Snippet: Mouse CD4 (mCD4)/pVax was constructed by PCR amplification of pBS mCD4 (Addgene, Cambridge, MA) using primers 5′-CCCCCGAATTCCACCATGTGCCGAGCCATCTCTCTT-3′ and 5′-CCCCCCTCGAGTCAGATGAGATTATGGCTCTT-3′, which contain the EcoRI and XhoI restriction sites.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Staining, Control, Transfection, Plasmid Preparation

Results of fine-mapping the ibalizumab epitope by point mutations in the chimeric mCD4-hGACFG (A) or hCD4 (B). Amino acids in regions of interest for ibalizumab binding were mutated to alanine, glycine, or the corresponding mouse amino acid as shown by single-letter amino acid symbols above the CD4 sequence alignment. Mutations with no impact on CD4 expression or ibalizumab binding are unmarked. Residues critical to ibalizumab binding are enclosed by boxes. A gray dot denotes the amino acid that partially affected ibalizumab binding. The asterisks indicate mutations that resulted in a substantial reduction (to <25%) in CD4 expression.

Journal: Journal of Virology

Article Title: Epitope Mapping of Ibalizumab, a Humanized Anti-CD4 Monoclonal Antibody with Anti-HIV-1 Activity in Infected Patients ▿

doi: 10.1128/JVI.00453-10

Figure Lengend Snippet: Results of fine-mapping the ibalizumab epitope by point mutations in the chimeric mCD4-hGACFG (A) or hCD4 (B). Amino acids in regions of interest for ibalizumab binding were mutated to alanine, glycine, or the corresponding mouse amino acid as shown by single-letter amino acid symbols above the CD4 sequence alignment. Mutations with no impact on CD4 expression or ibalizumab binding are unmarked. Residues critical to ibalizumab binding are enclosed by boxes. A gray dot denotes the amino acid that partially affected ibalizumab binding. The asterisks indicate mutations that resulted in a substantial reduction (to <25%) in CD4 expression.

Article Snippet: Mouse CD4 (mCD4)/pVax was constructed by PCR amplification of pBS mCD4 (Addgene, Cambridge, MA) using primers 5′-CCCCCGAATTCCACCATGTGCCGAGCCATCTCTCTT-3′ and 5′-CCCCCCTCGAGTCAGATGAGATTATGGCTCTT-3′, which contain the EcoRI and XhoI restriction sites.

Techniques: Binding Assay, Sequencing, Expressing

Results of fine-mapping of the M-T441 epitope by point mutations in the backbone of hD2-mCD4 (A) or hCD4 (B). Unmarked mutations have no impact on CD4 expression or M-T441 binding. Mutations enclosed by boxes substantially decreased M-T441 binding.

Journal: Journal of Virology

Article Title: Epitope Mapping of Ibalizumab, a Humanized Anti-CD4 Monoclonal Antibody with Anti-HIV-1 Activity in Infected Patients ▿

doi: 10.1128/JVI.00453-10

Figure Lengend Snippet: Results of fine-mapping of the M-T441 epitope by point mutations in the backbone of hD2-mCD4 (A) or hCD4 (B). Unmarked mutations have no impact on CD4 expression or M-T441 binding. Mutations enclosed by boxes substantially decreased M-T441 binding.

Article Snippet: Mouse CD4 (mCD4)/pVax was constructed by PCR amplification of pBS mCD4 (Addgene, Cambridge, MA) using primers 5′-CCCCCGAATTCCACCATGTGCCGAGCCATCTCTCTT-3′ and 5′-CCCCCCTCGAGTCAGATGAGATTATGGCTCTT-3′, which contain the EcoRI and XhoI restriction sites.

Techniques: Expressing, Binding Assay